It frequents arid or semi-arid locations. The Pallid Bat is usually found in rocky, mountainous areas and near water. They are also found over more open, sparsely vegetated grasslands, and they seem to prefer to forage in the open. The Pallid Bat has three different roosts. The day roost is usually in a warm, horizontal opening such as in attics or rock cracks, the night roost is usually in the open, near foliage, and the hibernation roost, which is often in buildings, caves, or cracks in rocks (Miller 2002).The diet of this bat includes a significant proportion of beetles, grasshoppers, and moths, also it consumes scorpions and flightless arthropods such as crickets. Lizards have also been reported as prey items. Mating takes place in late autumn or early winter. Female Pallid Bats store sperm in the reproductive tract until ovulation takes place in the spring. Births generally occur in large maternity colonies in May and Junes. Males are generally absent from these maternity colonies. Yearling females have single offspring, whereas older females may have twins annually (Wilson and Ruff 1999).

This species occurs in Queretaro and Baja California (Mexico) to Kansas (USA) and British Columbia (Canada), it is also present in Cuba (Simmons 2005).

It is critical to implement managing strategies at some parts of its range, where the habitat may be subject to direct human disturbances.