Myotis evotis is insectivorous. This bat is found in a wide range of habitats, but is most commonly found in mixed coniferous forests, from humid coastal areas to montane forests. In southern British Columbia, long-eared myotis roost in tree cavities in dense forests. In adapting to forest management in certain areas of British Columbia, they have recently been found to roost in the stumps of clear-cut stands. Long-eared myotis prefer the stumps of ponderosa pine and lodgepole pine in these areas. In the large uninterrupted forests of the Pacific Northwest, M. evotis uses large snags for day roosts. These bats usually prefer snags that reach high into or above the forest canopy. In the badlands of the South Sasketchawan River Valley in Alberta, M. evotis are mostly found roosting in the crevices of sandstone boulders. Other places which function as day roosts are abandoned buildings, cracks in the ground, caves, mines, and loose bark on living and dead trees. (Chruszcz and Barclay 2002, 2003, Manning and Jones 1989, Nagorsen and Brigham 1993, Vonhof and Barclay 1996, 1997, Waldien et al. 2000).

This small bat is found from southern British Columbia, Southern Alberta, Southern Saskatchewan (Canada) to New Mexico (USA) and Baja California (Mexico) (Simmons 2005). Elevation ranges from sea level on the Pacific Coast to 2,830 meters in the mountains of Wyoming.

This species occurs in several protected areas through its geographic range. It is included inside the Mexican regulation for species conservation NOM-59-SEMARNAT-2001 (Arroyo-Cabrales pers. comm.).