The Evening bat lives in eastern deciduous forests, it inhabits elevations from sea level to 300 m. This species is a forest-dwelling species that roosts in tree crevices and behind loose bark, as well as in buildings. This species rarely enters caves but does participate in swarming activities at some cave entrances in late summer. They historically used tree hollows, but as forests have been cut, many have moved into wooden buildings (Watkins 1972). Maternity colonies are known from attics, the lofts of old barns, and hollow trees (Wilson and Ruff 1999). Evening bats leave their roost near dusk, and the individuals begin flying at a height of 12 to 23 m, but as darkness falls they come much lower to the ground. They have a slow and steady flight; during feeding maneuvers, the tail and wing membranes are used to capture and restrain prey (Linzey and Brecht 2005).
In Florida, these ats have been observed roosting beneath palm fronds in winter, suggesting that they may overwinter in warmer climates (Watkins 1972). Female migrants arrive at maternity roosts in late April and early May; males do not accompany females on northward migrations, instead they remain in southern latitudes during summer months (Wilson and Ruff 1999). Mating probably occurs in late summer and early fall, with the sperm being stored in the uterus of the female during the winter. Ovulation and fertilization occur in the spring. Females give birth to 1 to 3 pups (usually twins) during June. The average life span in the wild is probably about two years, although there are records of some individuals surviving for over five years (Watkins 1972).

This species is found from northern Veracruz (Mexico) to Nebraska, the Great Lakes, and Pennsylvania, south to Florida and the Gulf coast (USA) (Simmons 2005).

It is important to identify and monitor some potential threats at local scale. As a first step, reduce or avoid habitat loss.