A typical inhabitant of subtropical forests but found in a variety of habitats in various geographic regions. Found in tropical moist forests, but also may occur in subtropical moist or dry forests. It inhabits swamp forests, evergreen forests, and yards of dwellings (Handley 1976). Eumops glaucinus is frequently found in urban areas throughout its range (Brand 2002). Each colony of E. glaucinus consists of one male and several females. Members of this species become inactive in cooler climates but they are not known to hibernate. E. glaucinus is carnivourous insectivore (Brand 2002). Found to inhabit holes in trees (Barquez et al. 1993).

This species is found in South America, east of the Andes (McDonough et al. 2008, Baker et al. 2009), from Colombia and Venezuela to Guyana, Bolivia, Paraguay, northern Argentina and Brazil. Southern portion of western distribution expanded (Barquez et al. 2006). Not confirmed in Ecuador or Peru.

Found in several protected areas through its ample geographic range.