The family Leptosphaeriaceae is a group of fungi within the order Pleosporales of the class Dothideomycetes. Members of this family are primarily plant-associated, including both pathogens and saprophytes, commonly found on stems, leaves, or dead plant material. They are characterized by producing perithecioid or pseudothecial fruiting bodies with bitunicate asci and often dark, septate ascospores. Species in Leptosphaeriaceae can cause leaf spots, stem cankers, or other diseases in a variety of host plants, making them ecologically and economically significant. Their life cycle often includes both sexual reproduction via ascospores and asexual stages producing conidia, which aid in dispersal and infection.