Anoura geoffroyi is strongly associated with streams within tropical evergreen forest (Handley 1976, Emmons and Feer 1997, Reid 2009). It is considered insectivorous and facultative nectarivorous throughout its range (Gardner 1977). It feeds on nectar, fruit and pollen, and may visit flowers primarily to obtain insects (Willig et al. 1993). Plant species visited in Mexico include Agave, Ceiba, Calliandra, Eucalyptus, Ipomoea, Pinus and various composites (Ortega and Alarcon D. 2008). It roosts in small groups in caves, tunnels or tree hollows and is found in lowland rainforest, deciduous forest, gardens and plantations. A colony of about 75 was found in a tunnel in Peru, grouped into several small clusters and hanging from the ceiling (Tuttle 1970). It is common only where there are caves or rock crevices, and  rare or perhaps absent from lowland Amazonian forests lacking high ground, rocks and caves. A single birth peak occurs each year, late in the wet season (Wilson 1979).

This species occurs in Peru, Bolivia, southeast Brazil, the Guianas, Suriname and Ecuador to Tamaulipas and Sinaloa (Mexico). It is also found on Trinidad and on Grenada (Lesser Antilles) (Simmons 2005, Ortega and Alarcon-D. 2008). In Nicaragua, it only occurs in central and northern regions, in high areas (A. Medina pers. comm.). Records from Argentina were misidentifications of A. caudifer (Barquez et al. 1993). Taxonomic definition of the species has changed by the recent recognition of discrete morphological differences among Andean populations from Bolivia, Peru, Ecuador and Colombia and those from the lowlands of eastern South America (Mantilla-Meluk and Baker 2010). However, the status of all these populations has not been comprehensively established, and at the present the distribution of A. geoffroyi remains unchanged.

It occurs in a number of protected areas throughout its range. Recommended conservation actions include the identification and protection of caves where this species is found. Also, further research on the taxonomic distinction among putative species and subspecies of this species-complex should be enforced (Jarrin and Kunz 2008).