The natural history of the genus Chiroderma is poorly known (Peracchi et al. 2006). This species was thought to be found only in rainforest habitats (Bordignon 2005), but it occurs in a variety of habitats such as primary and secondary forests, small forested fragments, cultivated areas (Faria 1995) and even urban parks (Esbérard et al. 1996). Use of human-dominated areas indicates some flexibility of C. doriae to colonise or at least forage in urban areas if its food resource is available (Nogueira and Peracchi 2002). These records of C. doriae in disturbed areas are only from Rio de Janeiro (Esbérard 2003). Information about roosts is scarce (Peracchi et al. 2006). Species of Chiroderma are considered predominantly frugivorous. Some data suggest that pregnancy and lactation of C. doriae are seasonal events (Peracchi et al. 2006).

This species occurs in Minas Gerais and São Paulo (southeast Brazil) and Paraguay (Simmons 2005).

The recommended conservation action is to avoid habitat loss.